raybio® human cytokine antibody array v membranes  (RayBiotech inc)

Journal: PLoS ONE

Article Title: Mechanisms Involved in Alleviation of Intestinal Inflammation by Bifidobacterium Breve Soluble Factors

doi: 10.1371/journal.pone.0005184

Figure Lengend Snippet: A. After 4 hour-incubation of HT29-19A cells with TNFα± Bb (MOI 100) or Bb -CM, epithelial cell supernatants were tested by the Raybio® human cytokine array V (One representative array of two experiments). Secretion of chemokines (CXCL1, CXCL8, CCL4) and of other inflammatory molecules was inhibited in the presence of Bb and Bb -CM as judged by densitometric analysis. B. Long lasting inhibition (up to 7 hours) of CXCL8 secretion by Bb -CM (∼50%) was observed using ELISA. n = 7, * p <0.01 vs TNFα. C–D. HT29-19A cell monolayers were treated (4 hours) from the basolateral compartment with TNFα together with Bb or commensal bacteria ( B.breve ATCC 15698, L.rhamnosus 10863, E.rectale L15) placed in the apical compartment at increasing MOI. As quantified by ELISA, Bb dose-dependently inhibited TNFα-induced CXCL8 secretion, in contrast to other bacteria. n = 11, † p <0.0001 ; * p <0.001 vs TNFα.

Article Snippet: RayBio® Human Cytokine Antibody Array V membranes (Raybiotech Inc) were incubated with HT29-19A conditioned media and processed according to the manufacturer's instructions (Scheme of the various cytokines/chemokines detected can be found at http://www.raybiotech.com ).

Techniques: Incubation, Inhibition, Enzyme-linked Immunosorbent Assay

Journal: BMC Cancer

Article Title: Inhibition of macrophage migration inhibitory factor decreases proliferation and cytokine expression in bladder cancer cells

doi: 10.1186/1471-2407-4-34

Figure Lengend Snippet: Secreted cytokine array. Secreted proinflammatory cytokines down-regulated by HA, anti-MIF antibody and MIF anti-sense. Integrated density values (IDV, average intensity times total area) were determined for each individual cytokine dot. Individual cytokine dot intensities were normalized to HRP-positive controls on each membrane. Mean control data for each cytokine spot are expressed as protein ratios (cytokine specific band integrated density value divided by HRP-positive control integrated density value). Treatment data are expressed as +, greater than 2 fold increase in secretion compared with control cytokine spot; -, greater than 2 fold decrease in secretion compared with control cytokine spot; (-), decreased beyond detection.

Article Snippet: An array for secreted human cytokine proteins (Human Cytokine Array V, RayBio, Norcross, GA) was used to determine relative changes in secreted cytokines.

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: Skin telocytes versus fibroblasts: two distinct dermal cell populations

doi: 10.1111/jcmm.12671

Figure Lengend Snippet: Cytokine profiles of human skin telocytes and fibroblasts. Supernatants of these two kinds of cells were subjected to cytokine antibody array. (A) It presents the results of the array, every black dot represents a cytokine. (B) It is the list of cytokines, each being homologue for every black dot in A. POS: positive; NEG: negative; ENA: epithelial cell-derived neutrophil attractant; GCSF: granulocyte colony-stimulating factor; GM-CSF: granulocyte–macrophage colony stimulating factor; GRO: growth regulated oncogene; I-309: eosinophil chemo attractant protein CCL-1; IFN: interferon; MCP: monocyte chemotactic proteins; MCSF: macrophage colony -stimulating factor; MDC: macrophage-derived chemokine; MIG: monokine induced by gamma interferon; MIP: macrophage inflammatory protein; RANTES: regulated on activation normal T cell expressed and secreted; SCF: stem cell factor; SDF: stromal cell-derived factor; TNF: tumor necrosis factor; EGF: epidermal growth factor; VEGF: vascular endothelial growth factor. IGF: insulin-like growth factor; IGFBP: insulin-like growth factor binding proteins; PDGF-BB: platelet-derived growth factor-BB; BDNF: brain-derived neurotrophic factor; BLC: B-lymphocyte chemoattractant; CK: chemokine; FGF: fibroblasts growth factors; Flt: fms-like tyrosine kinase; GCP: granulocyte chemotactic peptide; GDNF: glial cell line-derived neurotrophic factor; HGF: hepatocyte growth factor; IP: interferon-gamma-inducible protein; LIF: leukemia inhibitory factor; MIF: macrophage inhibition factor; NAP: neural antiproliferative protein; IL: interleukin; NT: neurotensin; PARC: pulmonary and activation-regulated chemokine; PIGF: placental growth factor; TARC: thymus and activation regulated chemokine; TGF: transforming growth factor; TIMP: tissue inhibitor of metalloproteinases.

Article Snippet: By RayBio Human Cytokine Antibody Array V analyses performed on the supernatant from separately cultured TCs and Fbs, we detected the cytokine profile of both cell types, individually.

Techniques: Ab Array, Derivative Assay, Activation Assay, Binding Assay, Inhibition